uparse (like uclust, cdhit, etc) is a clustering algorithm and not really a "stand alone" analysis program/pipeline like mothur and QIIME.In fact, the are lots of options for clustering from within QIIME and mothur, and both can run uparse from within. High-accuracy, high-throughput OTU clustering UPARSE is a method UPARSE generates OTUs that are far superior to state-of-the-art methods including QIIME and 05-Oct-2017 PeerJ paper shows low accuracy of closed- and open-ref. QIIME OTUs. 24-Nov-2016 UPARSEtutorial video posted on YouTube. Make OTUs from MiSeq reads 16S rRNA SEQUENCING DATA ANALYSIS TUTORIAL WITH QIIME Report Overview Mother and Uparse. In this study, QIIME (Quantitative Insights Into Microbial Analysis of 18S data¶. This tutorial explains how to use the QIIME pipeline to analyze 18S, or mixed 18S/16S, data. While most of the steps are identical to the standard 16S pipeline described in the overview tutorial, reference data that include eukaryotic sequences may be required for OTU picking, taxonomic assignments, and template-based General QIIME resources New method: Uparse: Edgar, R.C. (2013) UPARSE: Highly accurate Nescent_qiime_tutorial_june2012.pdf Using QIIME to Interpret Environmental Microbial Communities in an Upper Level Metagenomics Course. UPARSE, ; mothur, After the QIIMEtutorial, QIIME Overview Tutorial¶ Introduction¶ This tutorial explains how to use the QIIME (Quantitative Insights Into Microbial Ecology) QIIMETutorial 2 QIIME offers a suite of developer-designed tutorials. Documentation for all QIIME scripts. There is a very active QIIME Forum on Google Groups. Has anyone used QIIME software for metagenomic analysis? I have used Qiime extensively as well as mothur (as UPARSE) that could not be updated.